Three-hour discussion on the design aspects:

Update so far:

• Water body - Local/inland - river systems/ponds etc.
• Solution - Microfluidic-based impedance spectroscopy
• Size range of microplastics that we would like to detect - still to be decided
• Is microfluidic-based solution scalable?
• For a start, should we use a standard sample so that we know for sure there is enough microplastics in the sample?
• Do we need to some sort of filtration before the sample enters the microfluidic chamber?
• Standard impedance values expected for different microplastics? Any numbers that we can extract from previous studies?
• A stop- and- go well type impedance sensor: evaluation of electrode placement- parallel/ coplanar/ coaxial - geometric parameters and their effects?
• Equivalent model for the circuit- how do we tune the characteristic time?
• Signal generator by V & A multiplication of aux output on laptop/smartphone/ Arduino - evaluate possibility
• Flow control contraptions- hand pump/ motor etc.: come up with designs
• Deconstruction of FACS system
• Do we need sample aggregation? If the impedance data is equivalent to volumetric occupancy of microps, what is the number of samples to be taken for a statistically valid estimate (we can begin working on the framework)
• Standard protocol for MicroP preparation
• Can we make calibration worthy samples with microbeads in RO water?